Thirty of the estates where the birds are raised and shot received £4 million (US$4.85 million) in public cash in 2014, including one owned by the Duke of Westminster.
The duke is the richest landowner in the UK and is worth an estimated £9 billion.
The investigation carried out by Friends of the Earth supports an argument by campaigners who say that grouse farming is damaging to the environment and uses far too much space.
MPs are due to debate the issue on Monday after 120,000 people signed a petition to ban the most common form of grouse shooting.
“These shocking new figures reveal the true, horrifying scale of grouse moors in England and the madness of the current farm payments system that subsidizes them,” Guy Shrubsole of Friends of the Earth told the Guardian Friday.
“Instead of handing out taxpayers’ money to billionaires and offshore firms to indulge in an elite sport, the government must reform farm payments so public money is spent on public goods – like tree-planting, restoring wildlife habitats, farming sustainably and preventing flooding downstream,” he added.
Other claims include that grouse farming can lead to the illegal killing of birds of prey and legally decimate the populations of stoats and foxes, all of which prey on grouse.